mewo human melanoma cell line  (ATCC)

Journal: Cell reports

Article Title: MITF, TFEB, and TFE3 drive distinct adaptive gene expression programs and immune infiltration in melanoma

doi: 10.1016/j.celrep.2025.116499

Figure Lengend Snippet: (A) Left, western blot of IGR37 melanoma cell line transfected with control or MITF-specific small interfering RNA (siRNA). Right, RT-qPCR for PTEN mRNA from corresponding IGR37 cells. (B and C) Heatmaps showing relative mRNA expression in CCLE melanoma or Tsoi et al. melanoma cell lines. (B) Right, Pearson correlation of gene expression. (D) TCGA melanomas ranked by MITF expression ( MITF ; black line). Gray bars indicate expression of RRAGD or FNIP2 in each melanoma, with the moving average of each per 20 melanoma window indicated by colored lines. (E) MITF ChIP-seq showing binding to the RRAGD or FNIP2 genes. (F) Western blot of melanoma cell lines. (G and H) Box and whisker plots showing relative expression of MITF , RRAGD , and FNIP2 based on triplicate RNA-seq of IGR37 cells expressing doxycycline-inducible β-catenin (iCTNNB1) (G) or in MeWo cells after short hairpin RNA (shRNA)-mediated depletion of MITF (H). See also .

Article Snippet: MeWo Human melanoma cell line (male) , Obtained from the ATCC , Cornil et al. .

Techniques: Western Blot, Transfection, Control, Small Interfering RNA, Quantitative RT-PCR, Expressing, Gene Expression, ChIP-sequencing, Binding Assay, Whisker Assay, RNA Sequencing, shRNA

Journal: bioRxiv

Article Title: Zebrafish Xenografts Reveal a Context-dependent Role of PFKFB4 in Melanoma Cell

doi: 10.1101/2025.09.06.674616

Figure Lengend Snippet: (A) Schematic representation of the zebrafish xenograft assay. (B) Migration capacity was assessed in MeWo-GFP or A375P-GFP cells. Starting 48h after siRNA transfection, cell migration was tracked by live microscopy for 16 h. Migration velocity was reduced in both cell lines following PFKFB4 knockdown with siRNA targeting PFKFB4 (siPFKFB4), compared with cells transfected with non-targeting control siRNA (siCtrl). Bar plots represent mean ± SEM of three independent experiments (unpaired t- test). Kernel density plots illustrate the distribution of migration speeds in siCtrl versus siPFKFB4 groups. (C) (E) Number of invasive MeWo-GFP foci (C) or A375P-GFP foci (E) per fish at 3 days post injection (dpi) after transfection with siCtrl or siPFKFB4. (D) (F) Representative zebrafish larvae at 3 dpi xenografted with MeWo-GFP (D) or A375P-GFP (F) cells after transfection with siCtrl or siPFKFB4. Asterisks indicate invasive foci detached from the primary tumor. Orange dotted lines mark the swim bladder boundary. (G) Number of distant metastatic A375P-GFP foci, defined as cells migrating > 300 μm from the tumor boundary. (H) Magnification of the tail region showing A375P-GFP cells metastasizing to caudal hematopoietic tissue. In panels C , E , and G , each dot represents one fish. Statistical significance was determined by unpaired t- test. n.s., not significant (P > 0.05); *P < 0.05; dpi, days post injection; dpf, days post fertilization.

Article Snippet: Human metastatic melanoma cell lines MeWo (ATCC #HTB-65) , A375M , and A375P-GFP (ATCC #CRL-3224), as well as HEK-293FT cells (Thermo Fisher), were maintained in complete RPMI-1640 medium (MeWo, A375M; Gibco, #21875-034) or in complete DMEM (A375P, HEK-293FT; Gibco, # 41965-039), supplemented with 10% fetal bovine serum (Eurobio, #CVFSVF00-0U) and 1% penicillin/streptomycin (Sigma, #P0781).

Techniques: Xenograft Assay, Migration, Transfection, Microscopy, Knockdown, Control, Injection

Journal: bioRxiv

Article Title: Zebrafish Xenografts Reveal a Context-dependent Role of PFKFB4 in Melanoma Cell

doi: 10.1101/2025.09.06.674616

Figure Lengend Snippet: (A) Protein expression levels of the EMT-TFs SNAIL2, SNAIL1, TWIST, ZEB2 and ZEB1 across different melanoma cell lines. (B) mRNA expression of EMT-TFs in MeWo cells assessed by qPCR 48h after transfection with siCtrl or siPFKFB4. Statistical analysis was performed using an unpaired t-test. (C, D) Normalized protein expression of EMT-TFs in MeWo (C) or A375M (D) cells, 48h after transfection with siCtrl or siPFKFB4. Each dot represents one biological replicate with paired values connected by lines. Statistical analysis was performed using a paired t- test. n.s, not significant (P > 0.05); *P < 0.05; **P < 0.01.

Article Snippet: Human metastatic melanoma cell lines MeWo (ATCC #HTB-65) , A375M , and A375P-GFP (ATCC #CRL-3224), as well as HEK-293FT cells (Thermo Fisher), were maintained in complete RPMI-1640 medium (MeWo, A375M; Gibco, #21875-034) or in complete DMEM (A375P, HEK-293FT; Gibco, # 41965-039), supplemented with 10% fetal bovine serum (Eurobio, #CVFSVF00-0U) and 1% penicillin/streptomycin (Sigma, #P0781).

Techniques: Expressing, Transfection

Journal: bioRxiv

Article Title: Zebrafish Xenografts Reveal a Context-dependent Role of PFKFB4 in Melanoma Cell

doi: 10.1101/2025.09.06.674616

Figure Lengend Snippet: (A) Mean migration velocity of MeWo cells 48 h after transfection with siCtrl or siRNA targeting SNAIL2 (siSNAIL2). Kernel density plots illustrate the distribution of migration speeds in control versus SNAIL2 knockdown groups. (B,C) Expression of PFKFB4 and SNAIL2 proteins (B) and mean migration velocity (C) of MeWo cells transfected with siCtrl or siRNA targeting PFKFB4 (siPFKFB4), combined with empty pcDNA vector or a plasmid expressing Xenopus laevis SNAIL2 (xSNAIL2). Kernel density plots show the distribution of migration speeds across groups. xSNAIL2 failed to rescue the migration defect induced by PFKFB4 knockdown. (D) Mean migration velocity of MeWo cells after siCtrl or siPFKFB4 transfection, combined with empty pcDNA vector or a plasmid expressing Xenopus laevis PFKFB4 (xPFKFB4). Kernel density plots show the distribution of migration speeds. xPFKFB4 was able to rescue the migration defect caused by PFKFB4 knockdown. (E, F) Protein expression levels of PFKFB4 and SNAIL2 (E) and quantification from four biological replicates (F) in MeWo cells transfected with siCtrl or siPFKFB4, combined with empty pcDNA or xPFKFB4 plasmid. xPFKFB4 failed to rescue the decrease in SNAIL2 protein levels caused by PFKFB4 knockdown. In panels A , C , and D , bar plots represent mean ±SEM and were analyzed using an unpaired t- test. (F) Each dot represents one biological replicate, with paired values connected by lines; significance was assessed using a paired t- test. n.s., not significant (P > 0.05); *P < 0.05.

Article Snippet: Human metastatic melanoma cell lines MeWo (ATCC #HTB-65) , A375M , and A375P-GFP (ATCC #CRL-3224), as well as HEK-293FT cells (Thermo Fisher), were maintained in complete RPMI-1640 medium (MeWo, A375M; Gibco, #21875-034) or in complete DMEM (A375P, HEK-293FT; Gibco, # 41965-039), supplemented with 10% fetal bovine serum (Eurobio, #CVFSVF00-0U) and 1% penicillin/streptomycin (Sigma, #P0781).

Techniques: Migration, Transfection, Control, Knockdown, Expressing, Plasmid Preparation